目的 采用核磁共振代谢组学技术对款冬茎和叶的化学成分进行比较。方法 对款冬茎和叶进行核磁共振化学分析,然后通过主成分分析(PCA)、偏最小二乘法判别分析(PLS-DA)、正交偏最小二乘法判别分析(OPLS-DA)等多元统计分析手段结合单变量分析确定款冬茎和叶的差异成分。结果 款冬茎和叶核磁共振图谱共指认出40个化合物,多元统计和单变量分析结果显示,款冬叶中缬氨酸、亮氨酸、异亮氨酸、脯氨酸、绿原酸、3,5-二咖啡奎尼酸、3,4-二咖啡奎尼酸、款冬酮等的含量高于茎,而α-葡萄糖、β-葡萄糖的含量低于款冬茎,款冬茎和叶之间无明显差异的化合物有苹果酸、蔗糖、胆碱等。结论 本实验从整体物质组成角度比较了款冬茎和叶部位的化学成分差异,研究结果可为款冬茎、叶部位的资源利用奠定基础。
Abstract
OBJECTIVE To compare the chemical compositions of the stems (STL) and leaves (LTL) of Tussilago farfara L. using NMR-based metabolomic approach. METHODS The STL and LTL were analyzed by NMR, then the differential components were determined by multivariate statistical methods, including PCA, PLS-DA, OPLS-DA, and univariate analysis. RESULTS Fifty compounds were tentatively identified in the NMR spectra of STL and LTL. Multivariate coupled with univariate analysis revealed that some metabolites, such as valine,leucine,isoleucine,proline,chlorogenic acid,3,5-O-dicaffeoylquinic acid, 3,4-O-dicaffeoylquinic acid, and tussilagone,were present at higher levels in the LTF, while the STF contained higher levels of α-glucose and β-glucose.For some metabolites, such as malic acid, sucrose and choline, no significant differences were observed between STF and LTF. CONCLUSION This study reveals the chemical differences between STF and LTF in a holistic way, and lays the scientific foundation for the resource utilization of the stems and leaves of T. farfara L.
关键词
款冬 /
核磁共振 /
代谢组学 /
多元统计分析 /
茎 /
叶
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Key words
Tussilago farfara L. /
1H-NMR /
metabolomics /
multivariate statistical analysis /
stems /
leaf
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脚注
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基金
国家自然科学基金项目资助(31270008);国家中药标准化项目资助(ZYBZH-Y-JIN-34)
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